After all the XMRV on ERV, as Wendy would say, “Im finished!”
At this point, there are innumerable lines of evidence suggesting that XMRV is not a real human pathogen– from its magical ability to be spread despite no virus in any body fluid, including blood, including in HIV-1 patients, to its inability to infect its ‘target cell’ while it infects other cells, to its refusal to mutate more than two nucleotides on its own or in response to human mutagens in vivo, except that it does in vitro, its inability to give rhesus macaques even the most menial symptoms after being administered at a should-have-probably-been-lethal dose, to the fact not one lab on the planet can reliably detect it in humans, to the fact ‘XMRV infected’ people dont make antibodies ‘to’ XMRV, while healthy controls do– For XMRV to be a real human pathogen, it literally has to be an alien time-traveler from another dimension with some sort of Romulan cloaking device and/or Harry Potters magic cloak thingie.
It makes a lot more sense that ‘XMRV’ is really a lab contaminant, and once you start ‘looking’ for a contaminant, you can find it (whether you want to or not).
But pro-XMRV labs did have one good leg left to stand on: They mapped XMRV integration sites in prostate tumors from ‘XMRV’ prostate cancers.
*nod* There you go. They didnt just find the virus or evidence the virus was somewhere (or was somewhere, at some point), they found *it* and *exactly* where it integrated.
Except, apparently they didnt.
Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection
XMRV is a gammaretrovirus associated in some studies with human prostate cancer and chronic fatigue syndrome. Central to the hypothesis of XMRV as a human pathogen is the description of integration sites in DNA from prostate tumour tissues. Here we demonstrate that 2 of 14 patient-derived sites are identical to sites cloned in the same laboratory from experimentally infected DU145 cells. Identical integration sites have never previously been described in any retrovirus infection. We propose that the patient-derived sites are the result of PCR contamination. This observation further undermines the notion that XMRV is a genuine human pathogen.
Oh for fucks sake.
Long time readers of ERV know what this means from my list of Creationist Claims about ERVs:
The first paper simply states that some retroviruses like to insert in genes, some like to insert near promoters of genes, and some like to insert in the middle of no where. The specific insertion sites, what base pairs on on the left, which ones are on the right, is random.
The odds of having the ‘same’ virus at the ‘same’ location in two independent organisms is fucking astronomical, to the point where we havent ever observed it. Ever. UNTIL XMRV, AMIRITE!! No. The ‘mapped integration sites’ are fucking contaminations from cell lines infected with XMRV.
For fucks sake.
It is striking that there have been no independent reports of patient-derived XMRV integration sites nor have there been any descriptions of polytropic or modified polytropic MLV integration sites in human samples despite the apparent detection of these viruses in CFS patients .
Hey.. hey guys. Why dont you all try to guess who  is. Ill give you a hint: It is Shyh-Ching Lo, Natalia Pripuzova, Bingjie Li, Anthony L. Komaroff, Guo-Chiuan Hung, Richard Wang, and Harvey J. Alter. The group who ignored their reviewers request for them to map their integration sites, because Alter is a NAS member, and they could. It is striking that they didnt ever do that, isnt it, Jeremy A. Garson, Paul Kellam, and Greg J. Towers?
Neither did Judy “NO ONE CAN FIND THE VIRUS BUT ME!!!!! I AM THE MESSIAH!!!” Mikovits.
But maybe not so striking considering how the prostate cancer lab ‘mapped’ their sites. ‘Normal’ PCR? Meh, 30-40 cycles, whether youre doing regular or real-time. Sometimes you need a second round of PCR to bump up your signal more, or nested PCR, doubling that.
Jeremy A. Garson, Paul Kellam, and Greg J. Towers generously called the labs protocol ‘unusual’:
The propensity for PCR contamination is increased due to the unusual technique used for cloning the prostate tissue-derived integration sites which involved an extraordinary degree of PCR amplification with 80 preliminary amplification cycles followed by nested PCR consisting of 29 cycles and then an additional 18 cycles . PCR tubes were opened during the procedure for the addition of fresh DNA polymerase after 40 cycles.
I do some weird PCR. I do. I have done some crazy ass shit to get my stuff to work, but I could tell you the exact biochemistry behind what I was doing, thus why I was doing it. And *I* think their method goes beyond ‘unusual’ and well into ‘convoluted and bizarre, to the point where I dont trust this data without further information’. And then theres this:
No negative controls were mentioned in the published method .
Oh for fucks sake.
I suppose I must give credit where credit is due– Mikovits was quoted as saying something recently at a conference hosted by (SHOCK!) a troupe of woo-peddlers, ‘Gordon Medical Associates’:
The subject I’ve stayed away from, that was discussed intermittently, was the politics of it all. Our government’s involvement, or lack thereof. I’d like to close with a quote from Mikovits, when asked about the politics and all the second guessing that has occurred regarding her research today. Very confidently, and quickly she retorted, “I think the politics will go away shortly.” Period. All she said. There was a gasp in the room, and she moved on as if she had just said “please pass me a glass of water.”
*shrug* She was right. Its over.
PS– Also, no XMRV associated with autism or families of autistic people. Still: