Ellman reagent can be used to quantitate the amount of free thiol present in a protein or other molecule:
It reacts with the free thiol and releases a nitrocarboxythiophenolate, which is bright yellow. You can then quantitate it with a UV-vis.
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Ellman Reagent (See your sulfur)
Category: Biology
Posted on: August 27, 2007 9:21 PM, by Molecule of the Day
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