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The author is not a physician. The content on this website does not, and is not intended to constitute medical advice. It should not be relied upon when making medical decisions. It is not intended as a substitute for advice from your physician or other healthcare provider.

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Ellman Reagent (See your sulfur)

Category: Biology
Posted on: August 27, 2007 9:21 PM, by Molecule of the Day

Ellman reagent can be used to quantitate the amount of free thiol present in a protein or other molecule:

InChI=1/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)


It reacts with the free thiol and releases a nitrocarboxythiophenolate, which is bright yellow. You can then quantitate it with a UV-vis.

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