Not Exactly Rocket Science

By directing the evolution of a worm, scientists have confirmed answers to the age-old question: “What is the point of having sex with someone else?” For most people, that would hardly be a tricky query but it’s no reflection on the lives of evolutionary scientists that sex has been one of biology’s oldest puzzles.

i-c8fc052265f5ed1da9969f62c6b97733-celegans.jpgThe problem is this: many creatures can reproduce by fertilising themselves instead of getting someone else to do it, and at first glance they should do much better individuals that cross-fertilise. For a start, they’d ensure that all of their genes reach the next generation, while mating with another individual reproduction halves their genetic legacy. And without having to find males, self-fertilising females should be able to produce twice as many offspring. This is the “two-fold cost of males”.

And yet, cross-fertilisation is the more common strategy in the animal world, so it must have advantages that compensate for its cons. Scientists typically name two. The first is that by shuffling the genes of two parents, cross-fertilisation deals the next generation with a fresh genetic hand, better equipping it to rapidly adapt to changing environments, predators and parasites. The second is that having sex with someone else prevents harmful mutations from building up (the genetic defects that plague inbred families would be even worse in lineages that only ever have sex with themselves). They’re the same reasons why sex itself is usually a better long-term solution than asexual cloning.

The problem is that both of these explanations have proven very difficult to test. But that didn’t stop Levi Morran and colleagues from the University of Oregon, who demonstrated that both justifications are correct, by manipulating the evolution of the nematode worm Caenorhabditis elegans.

Like humans, C.elegans has two sexes but unlike us, they are males and hermaphrodites (with males making up just one in every two thousand individuals).  Equipped with both sets of genitals, hermaphrodites worms can fertilise themselves without male help – far from being rude, telling C.elegans to go &$&! itself is a feasible lifestyle suggestion. Hermaphrodites could also mate with males, but they do that on less than one in 20 occasions.

However, the genetics of this animal are so well-known that Morran managed to use two mutations to create strains of C.elegans that either always had sex with themselves, or always had sex with other worms. Morran subjected these two engineered strains, as well as a normal one, to two challenges.

Some were exposed to a chemical called ethyl methanesulphonate (EMS) that quadruples the normal mutation rate of DNA, riddling their genomes with potentially harmful genetic changes. To make matters worse, they were placed in a new environment that should weed out all but the fittest individuals.

Despite these challenges, the strain that always mated with others were still successful after 50 generations and fared much better than the strain that only had sex with itself. Even the normal worms moved towards a cross-fertilising strategy under these harsh conditions. These are all signs that having sex with others else provides a way of purging harmful mutations from a population. By contrast, Morran estimated that the genetic burden carried by the worms that only ever mated with themselves would drive them to extinction after a few hundred generations.

Morran also exposed some of his worms to a bacterium called Serratia marcescens, a bacterium so virulent that it kills 80% of the worms it infects. It was a test of their ability to rapidly adapt to new challenges, and one that the cross-fertilisers passed with flying colours. They quickly evolved to resist the bacterium, while the populations that only mated with themselves did not. As before, the normal worms shifted towards a cross-fertilisation strategy when faced with the new threat.

So both theories are correct – compared to having sex with yourself, doing it with someone else provides a way of resisting harmful mutations and adapting quickly to new challenges. Morran says that species that evolve to always self-fertilise become trapped in an “evolutionary dead-end” and are “ultimately doomed to extinction”.

Reference: Nature doi:10.1038/nature08496

More on sex and reproduction:

Image: C.elegans by Bob Goldstein


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Comments

  1. #1 Karen James
    October 22, 2009

    Great post, Ed, but how do we know that the two mutations did not have an impact on fitness independent of the obligatory self- or cross-fertilization phenotype?

  2. #2 Lilian Nattel
    October 22, 2009

    Nice proof–but I do feel squirmy at messing with worms. They are still living creatures.

  3. #3 Nathan Myers
    October 22, 2009

    A squirmy feeling is precisely appropriate in this context.

    Morran’s conclusion seems too strong given the success of bdelloid rotifers.

  4. #4 arvind
    October 22, 2009

    So if you keep playing with yourself, you just go (down the evolutionary) blind (alley)? :-)

  5. #5 Michele
    October 22, 2009

    C. elegans hermaphrodites make sperm and then the gonad “switches” to producing oocytes. Interestingly, male sperm outcompete hermaphrodite sperm to fertilize oocytes. When a hermaphrodite mates, as long as the male sperm are normal, they will fertilize the eggs that pass through the spermatheca preferentially over hermaphrodite sperm. Also, if a hermaphrodite is not mated, it will produce around 300 fertilized eggs. If a hermaphrodite is mated, it will continue producing oocytes as long as there are sperm present in the spermatheca (and as a result fertilized eggs); the hermaphrodite will essentially “mate to death”, producing many more offspring than the unmated, self-fertilized hermaphrodite. I haven’t read the paper, but it would be interesting to see if the production differential between wild-type hermaphrodites never mated and wild-type hermaphrodites continually exposed to males is maintained in the mutant strains used.

  6. #6 Rich Wilson
    October 23, 2009

    And if two sexes are good, then why aren’t three or four better? Why has such a thing never evolved? Or has it?

  7. #7 Kapitano
    October 23, 2009

    Rich Wilson: if two sexes are good, then why aren’t three or four better?

    Oh I know that one!

    Imagine a species has three sexes, and comes in two colours – red and green. It takes three individuals to reproduce, each contributing a random third of its DNA.

    If two of each “triad” are red and the other green, there’s a 2/3 chance the offspring will be green. If the offspring mates with another green and a red, the same probabilities apply, and after only a few generations, there may be no reds left at all.

    Having three sexes can actually reduce the genetic variability of a population, leading to the same problems as having only one sex.

    I’m sure Mr Yung can explain it much better, but as I understand it, that’s what it comes down to.

  8. #8 Nathan Myers
    October 24, 2009

    Some plants have been noted with as many as 17 sexes, varying according to how they are counted.

  9. #9 DJ
    October 24, 2009

    I believe you mean polyploidy, which is simply more than 2 sets of homologous chromosomes. It isn’t really indicative of more than 2 sexes though. (Maybe I’m wrong, but I’ve never heard of plants with 17 sexes before)

    What is really cool about polyploidy in plants is the immediate divergence to a new species that often accompanies it. Evolution is so cool.