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Thoughts and Findings Related to Neurobiology Lab
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Posted on: September 28, 2007 12:24 PM, by Blue_Expo
As one of my fellow classmates has already described in part, we have proposed to study the effects of sleep deprivation and alcohol on zebrafish. We have a good idea of how to execute experimentation on this topic. The meaning behind it however remains, as of yet, a bit vague. The idea was brought up during our last class discussion that we could experiment with the effects of sleep deprivation and alcohol on zebrafish development or adult mating and feeding patterns. We also thought of experimenting with the behavior of zebrafish on cocaine, or with the effects of alcohol and sleep deprivation on oursleves but neither of those ideas flew too high with PZ. Understandably so.
Although, while researching, I didn't find too many articles on intoxicated and sleep deprived zebrafish, I did find a lot of articles on genes and regeneration. In one experiment, researchers surgically removed a small portion of zebrafish hearts and then monitored recovery. The zebrafish were not only able to regenerate the removed portion of their hearts within two months, the regenerated heart tissue functioned the same and looked histiologically the same as the heart tissue of zebrafish in the control group (Poss, Wilson, & Keating). Another group of researchers discussed the effects of cyclic adenosine monophosphate on neuron regeneration in zebrafish (Bhatt, Otto, Depoister, & Fetcho). This could obviously have significant implications on human neuron regeneration.
After thinking about my findings for a moment, I discovered the reason why articles about regeneration out number articles on the effects of sleep deprivation and alcohol. Areas of research like regeneration are much more useful, not as well understood, and provide lots of room for scientific advancement. I'm not sure that I have the skill or tools required to surgically alter an organism the size of a minnow or track specific chemicals throughout the nervous system but I'll definately be giving some thought to possibilities for regeneration experiments over the weekend. If anyone has any suggestions, I would be pleased to read them.
References:
Kenneth D. Poss, Lindsay G. Wilson, Mark T. Keating. Heart Regeneration in Zebrafish. Science. 13 December 2002. Vol. 298. no. 5601, pp. 2188 - 2190
Dimple H. Bhatt, Stefanie J. Otto, Brett Depoister, Joseph R. Fetcho. Cyclic AMP-Induced Repair of Zebrafish Spinal Circuits. Science. 9 July 2004. Vol. 305. no. 5681, pp. 254 - 258
Comments
One big question in regards to your proposed research: how do you tell when a zebrafish is sleeping? Do they have a regular diurnal rhythm behaviorally or biochemically?
On another note, perhaps it's just me but when I hear of zebrafish and alcohol combined I keep picturing cliche`d frat hazing and swallowing the fish with the acohol, a decidedly un-scientific approach.
Posted by: Shaun | September 28, 2007 1:54 PM
Posted by: H. Humbert | September 28, 2007 2:03 PM
As PZ should know, fins clipped from adult zebrafish to make genomic DNA usually regenerate within a month. With MS222, this is an ethically sound procedure that doesn't seem to cause much suffering and quantitative data can be easily gathered.
Good luck,
Posted by: caynazzo | September 28, 2007 2:06 PM
Are you sure that college is not an experiment in sleep deprivation and alcohol on the students?
Posted by: Christian Burnham | September 28, 2007 2:19 PM
My guess: The Z-fish will start obsessing about Ron Paul.
Posted by: Christian Burnham | September 28, 2007 2:22 PM
How are you going to sleep-deprive fish? Search Google Scholar for work by Greg Cahill, Carla Green and/or Keith Barrett + zebrafish for some ideas on techniques for observing sleep-wake cycles in zebrafish.
Posted by: Coturnix | September 28, 2007 2:41 PM
Remember:
Being your own subject for alcohol and sleep deprivation experiments is OK.
For the regeneration studies...not so much.
Posted by: Dumb Guy | September 28, 2007 2:46 PM
Christian - shhhh. If they realise, they'll demand payment.
Bob
Posted by: Bob O'H | September 28, 2007 3:03 PM
If you're interested in regeneration, you might also read up on Ellen Heber-Katz, G. Masinde, X. Li, and others who have been working with MRL mice. Excellent heart tissue regeneration, among other things. Regenerative medicine is a promising field.
Some other names of potential interest regarding mamalian regeneration that I've jotted down: Alan Russell, Stephen Badylak and Hans Georg-Simon.
But re: zebrafish? I have no clue.
Posted by: Spaulding | September 28, 2007 3:05 PM
If you need background information, I'd be happy to write a paper on the effects of alcohol and sleep deprivation on me.
My guess is that you'll observe your subject zebrafish putting their fins around each other and loudly proclaiming, "This fish, is THE FISH. No, shhh, I gotta say something. This fish here, no, shhh, listen! This fish, is THE FISH, and I'm not jus' saying that, y'know wha' I'm saying?"
Posted by: Brownian | September 28, 2007 3:17 PM
Forgive my ignorance, but how does one feed a fish alcohol? Do you just spike the tank or something?
Posted by: kellbelle1020 | September 28, 2007 3:19 PM
As I recall from the protocol section of a paper on sleep deprivation in mice (which I think was the same one that was investigating the protein-folding differences that resulted from sleep dep), they simply got grad students to brush the mice with the hairs of a small paintbrush whenever the mice looked like they were dozing off.
Poor mice... "Can't sleep. Brush will get me."
And then they kill them and examine their brains.
Regeneration sounds interesting. How well is it understood? Are the same proteins and gene networks involved as in embryonic development?
Posted by: Owlmirror | September 28, 2007 3:23 PM
Alex Schier's lab at Harvard has also looked at sleep in zebrafish. Here's a recent paper-
Prober, DA et al. (2006) Hypocretin/orexin overexpression induces an insominia-like phenotype in zebrafish. J. Neurosci. 26, 13400-13410.
Posted by: Barn Owl | September 28, 2007 3:24 PM
If you're into regeneration, the obvious way to cross interests with sleep deprivation, alcohol and regeneration is to make four controls, one sleep deprived and drunk, one only sleep deprived, one only drunk, and one normal, then injure them in some regeneration-requiring way. If you want controls for THAT, you can re-split your four groups into "surgically injured" and "not injured", which would give you eight total groups, which is probably too much if you want a statistically significant number in each group (unless you have a lot of them, I guess).
Also, if you do manage to con cocaine off of PZ, take pictures.
Posted by: Dan | September 28, 2007 3:27 PM
Since you are thinking of young zebra fish, an interesting experiment would be to study the effects of stress (which could include sleep deprivation) on their growth rate. You mght need three groups - high stress, moderate stress and control. You could also try the same groups with regeneration. There is evidence that extreme stress leads to dwarfism with humans, and any continuous stress normally hampers growth of new tissue. But I doubt either of these things has ever been done with fish.
Posted by: sailor | September 28, 2007 4:51 PM
Of course, you might find it difficult to come up with a control group of well-rested, sober graduate students. There must be one out there somewhere...
Posted by: Mike O'Risal | September 28, 2007 4:58 PM
Wow. I'd like to see a successful fish surgery. Fish are so delicate and die so easily. Plus, fish surgery would have to be underwater.
Sorry, I know there's more to the article than fish surgery but I'm sort of fixated on that alone, LOL
Posted by: k | September 28, 2007 6:10 PM
While doing a simple dissection of Amphioxus in Vertebrate Biology lab, I soon abandoned the scalpel for a sharp pin. But if you're actually doing research, you might want to look into drawing some very tiny blades from glass.
Posted by: Monado | September 28, 2007 8:57 PM
Mike O'Risal,
No, there aren't.
Posted by: Melanie | September 28, 2007 9:17 PM
We already know what happens to physicists when they're sleep deprived or intoxicated: they turn into biology students! :D
Posted by: Kimpatsu | September 28, 2007 9:33 PM
I did "brain surgery" on butterflies years ago. We used fragments on glass from the em microscopy lab and glued them to thin wooden dowels. Super sharp and cheap.
Posted by: Steve | September 29, 2007 7:14 AM
Great blog, keep it going !
BO
Posted by: Bovis | September 29, 2007 8:25 AM
I'm with K on this one. Surgery on a fish to remove part of it's heart just blows my mind. Would anybody mind providing a small description of that procedure?
Posted by: Jim | September 29, 2007 12:48 PM
I have a fantastic idea! Why not experiment with leaving those poor little zebra-fish, who have not, and could not possibly, hurt you, alone? I predict if you don't feed them alcohol or slice off pieces of their little fish hearts off and change their water pretty regular, they will live long and happy lives. Try it.
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Posted by: Jimmy | September 30, 2007 4:02 PM
Successful
Thanks...
Posted by: Migen | December 4, 2007 9:34 PM