Alex Palazzo is a postdoctoral fellow working in the Department of Cell Biology at Harvard Medical School.
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Category: Lab Life
Posted on: March 5, 2008 7:54 PM, by Alex Palazzo
Each row of colonies represents one yeast strain lacking one gene from the Saccharomyces cerevisiae genome. There are 8 rows per dish, and 550 dishes, resulting in 4400 strains. Saccharomyces cerevisiae has about 6000 coding genes. Strains lacking essential genes are not included for obvious reasons.
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Comments
As my wife asked me on our first date, "Is it really that small?"
She didn't really say that, but looking at that visual rep of any genome makes it seem so tiny and inconsequential.
Ok...time for more bourbon.
Posted by: Jeb, FCD | March 5, 2008 9:15 PM
You could also use the Tags in the place of each ORF and screen your phenotype in the whole population of deletions at the same time.
Posted by: matt | March 5, 2008 10:32 PM
What about 46 96-well plates instead?
Posted by: sparc | March 5, 2008 11:22 PM
Nah, I like it old school! (I'm serious).
Toothpick power!
Posted by: Pinko Punko | March 6, 2008 3:15 AM
The postdoc who performed this screen was going to use a pipetting machine to plate out a modified collection of yeast knockouts. Unforetunately he ran into problems and had to plate the cells manually.
Posted by: apalazzo | March 6, 2008 8:00 AM
I hope they are sealed with a parafilm or something, otherwise I can't even imagine how the room/fridge smell :|
Posted by: Arman | March 7, 2008 10:29 AM