Sphingolipid Synthesis

I’ve spent this last week familiarizing myself with this article for my biochemistry class. Obviously, the article is way to large to bite off in one blog. One spot that draws my curiosity.

The AUR1 is promoted by the presence of Galactose. The kicker is that the presence of Glucose will turn off the gene. The organism is unable to live without the target sphingolipids. Is there some reason for this? I would think that adaptation would have long since accounted for this. Weird.


  1. #1 minimalist
    December 7, 2007

    Yeah, it’s much more common in yeast though. The GAL inducible promoter is a great tool for overexpressing proteins (for purification or phenotypic effects), or even tracking its movement through the cell: say you fuse GFP to your protein of interest and put it under the promoter. Where does it end up after 5, 10, 60, 1800 minutes of expression? Good way to characterize transport systems.

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