- Slowing down the DNA for long enough to read each base;
- Manufacturing consistent holes in a solid state medium - something that is extremely hard at a nanoscale (other current nanopore approaches instead use biological nanopores that are sturdy, cheap to produce using bacteria, and highly consistent);
- Reading each base to the accuracy required for an industrial sequencing technology.
You might want to add that one aspect of this is the bioinformatics tools needed to process and analyse the data. This may also be an aspect that IBM may be aiming to address, too. In many ways, the algorithms needed to process and analyse the data are just as much of a problem as the hardware issues. Just a thought.
The assembly tools for nanopore sequencing are far simpler than for pyrosequencing approaches, as nanopore sequencers are expected to generate very long (100s to 1000s of kbp) reads...the annotation tools are the same used for other approaches, so I don't understand your point.
IBM was one of the first companies in the world to foray into âProject Checkmate,â in which IBM and The Scripps Research Institute proposed to conducted advanced biological research on influenza viruses in 2006! The effort involved using a powerful IBM Blue Gene Supercomputer to model the molecule hemagglutininâthe H in H1N1âin an attempt to predict the way flu viruses mutate.
Far as I know, the H1N1 pandemic began around April 2009.
Just makes ya go hmmmmmm.