I feel like I've seen this movie before. A group of thieves need to gain entry to a highly secured vault. The vault door is nearly impregnable, and once inside, there are motion sensors, security cameras and laser trip lines, all of which sound the alarm. When the security guards hear what's happening, they are told to release a deadly gas into the vault, killing anyone inside. But Salmonella enterica, that charming bug responsible for all manner of unpleasantness, is a clever burglar. It has learned to live inside macrophages - cells that are usually used to destroy bugs - and uses their own defenses against them.
The first step is getting inside, but when the cell is designed to eat bugs, this isn't much of a feat. Macrophages express a number of receptors that latch onto bacteria, viruses and all manner of dangerous things to pull them inside. But this thief wants to be caught, all it has to do is get near a macrophage, and it will be engulfed. The thieves just turn themselves in, knowing the guards will try to secure them in the vault. It's the next step that's tricky. Once macrophages engulf something, it is enclosed in a membrane-bound vesicle called a phagosome, which rapidly begins a transformation process. Everything inside is destined for destruction, so the phagosome begins to pump in protons, increasing the acidity. This is pretty inhospitable by itself, but acidification also activates enzymes designed to destroy any and all molecules they find.
Acidification itself is triggered by the receptors of the innate immune system, especially Toll-like receptors. TLR3, 7 and 9 have all been shown to contribute to clearing Salmonella. Not surprisingly, if you knock out one or two of these receptors, infection with these bugs is far more deadly. But researchers at UC Berkeley noticed something strange - if you knock out all three, mice are protected. In other words, if you disable the security cameras, motion sensors AND laser trip lines, the thieves can't finish the heist. How does that make any sense?
TLR Signaling Is Required for Salmonella typhimurium Virulence
For most bugs, macrophages spell doom, but Salmonella knows what to expect. It has evolved to express effector proteins that prevent the phagosome from getting too inhospitable. These effectors (encoded by a gene element called SPI-2) prevent activation of the degradative enzymes, and turn off the proton pumps to prevent the vesicle from becoming too acidic - they cut the gas lines to protect themselves. Other SPI-2 effectors make the vesicle more hospitable to growth. When one or two of the TLRs are disrupted, the rest of the immune response to bacterial infection is blunted, and since Salmonella is living in a hospitable vesicle of its own construction, it replicates like crazy.
The catch is that the trigger for Salmonella to release its SPI-2 effectors is when the phagosome starts to become acidic. Evidently, when TLR signaling is completely knocked out, there's no acidification, and the Salmonella just sits inside the vacuole. It doesn't realize that it's in the right location, so it behaves as if it's still trying to find a host. My metaphor might break down a bit at this point, but I'll try to press ahead anyway - the thieves don't hear an alarm, so they reason that they must not actually be in the vault.
Here's a fun little video in which the main authors explain the paper. I like the idea of science journals trying to do this sort of thing. I don't know if it appeals to a wide audience, but at least they're making an effort.
TLR signaling is required for Salmonella typhimurium virulence from Kevin Bonham on Vimeo.
Fun personal note: the second author on this paper is a first year in the Immunology Program here at Harvard, and is also an Olympic Swimmer. More importantly, I was his buddy during recruitment last year, so he's only here because of me.
Arpaia N, Godec J, Lau L, Sivick KE, McLaughlin LM, Jones MB, Dracheva T, Peterson SN, Monack DM, & Barton GM (2011). TLR Signaling Is Required for Salmonella typhimurium Virulence. Cell, 144 (5), 675-88 PMID: 21376231
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You may want to correct the spelling in the title of this article, unless it's deliberate and I missed the point.
Clever little bugs...
Re: your profile: Genetically unable to grow facial hair? Even my friend who had a sex change from female to male is growing facial hair! Sorry dude. But I was wondering, do they make beard "wigs"? You could do the professor thing yet!
@don - oops. Can't change the url at this point... oh well.
@k - Thanks. I should maybe be more clear - I can grow facial hair, it just takes forever and there are uneven patches on my chin and upper lip, but not much else. Look at my picture on the left there - that was me unshaven for about a week.
The movie is actually a pretty good idea. I wouldn't be surprised if I found this on JoVE. I think the trend to make short videos explaining research findings will continue.
Also, loved seeing the microbe plush pop up in various sections :P
It's interesting that they only look at intracellular TLRs when for Salmonella you usually think of TLR 4 and 5.
I think it makes conceptual sense - the effect is on lysosome acidification, so why not look at endosomal TLRs?
But also, remember that the stuff that gets published isn't necessarily the only thing they looked at. I'm pretty sure they noticed this phenotype in the MyD88/TRIF double knock-out, and then went backwards to try to identify which TLR's wer responsible.