Extract DNA In Your Kitchen

Using only a Beer Glass and a few other household items and ingredients.

More like this

A Plea for Peaceful Coexistence - The Pour Blog - NYTimes.com "Beer and wine are not in competition. Yet people in the wine business, who I assure you drink an awful lot of beer, don't often take it seriously as a beverage. And people in the beer business, perhaps in reaction to not-so-imaginary…
The January 12, 2007 issue of MMWR reports on cases of infant deaths due to (presumably) unintentional overdoses on cough and cold preparations. With this being the season that such products are most likely to be used, I thought I'd call attention to this. Many people do not realize that there are…
New York Academy of Sciences Children's Science Book Award Winner Vicki Cobb has a new volume called "See for Yourself: More Than 100 Amazing Experiments for Science Fairs and Projects (Second Edition)" Actually, it isn't entirely new. It's a second edition, but updated. This is for 10 year olds…
I’ll admit it: I’m a bit of a beer snob. I make no bones about it, I like my beer, but I also like it to be good beer, and, let’s face it, beer brewed by large industrial breweries seldom fits the bill. To me, most of the beer out being sold in the U.S., particularly beer made by Anheuser-Busch and…

I gotta run to the drugstore and get some isopropyl alcohol. Otherwise I've got all the other ingredients.

Now I know what the heat is for, to denature the proteins. Ahhhhh. Now once you've got the DNA separated out how do you separate the base pairs?

Well, Tony, separating the helix can be done with heat as well, which is why he talks about being careful to not get it too hot. If you wanted to amplify this DNA sample you'd be using PCR. That involves heating a sample up, to the point of unzipping the helix, in the presence of a solution containing the 4 base pair elements and a thermally stable enzyme that will zip up the helix. You heat, separating the strands, then cool, allowing the enzyme to zip the free floating bases onto your two strands. Then you heat and cool again to come up with 4 strands. Repeat until you have the concentration you want.

A lot of times you're only interested in a small chunk of the genome, so you use more enzymes to trim a section of the strand out, then work at duplicating that.

So, to do that at home, you'd need to order the appropriate enzyme(s), and a small supply of the 4 bases. I'd be surprised if those weren't readily available. I've seen electrophoresis/PCR kits for kids at Fry's Electronics.

Hmmm ... Onion-soap DNA milkshake ... [drools]

thats COOL!!
i wanna do it :)

By anonymous (not verified) on 08 Apr 2009 #permalink