Why Antibiotic Resistance Surveillance Must Incorporate Genetics

A recent paper examined if use of the antibiotic cotrimoxazole was correlated with resistance in three different bacterial pathogens, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. To quickly summarize, in one species, S. pneumoniae, resistance was correlated with use, whereas it was not for the other two species. While the study is fine for what it is, it inadvertently highlights a problem most surveillance systems have:

they don't incorporate genetic information.

Without genotypic information, we don't really know what happened. Is the correlation spurious, in that a strain which happens to be resistant, spread rapidly, or is this a selective response to antibiotic use? Previous studies (discussed here) have shown that change in antibiotic resistance is often not correlated with antibiotic use. Instead, changes in the frequency of resistance are due to the turnover in (replacement of) clones that appear to have little to do with antibiotic resistance (believe it or not, bacteria actually do things other than resist antibiotics...).

Note that I refer to clones--a group of closely related strains*. It's hard, if not impossible, to determine a clone without genetic information. Unfortunately, most surveillance systems don't collect genetic information, and, if they do, it's usually from a non-random subset (very small subset) of strains (there are exceptions; virtually all of these exceptional surveillance systems are federally funded).

Now, if there were only a microbiologist with access to a big ol' genome center who gave a damn about this....

*If this sounds vague, it is. Obviously, the resolution of the method, along with decisions by researchers, will determine which isolates belong to a clone.

Cited article: Pauliina Kärpänoja, Solja T. Nyberg, Miika Bergman, Tinna Voipio, Pirkko Paakkari, Pentti Huovinen, Hannu Sarkkinen, and the Finnish Study Group for Antimicrobial Resistance (FiRe Network). 2008. Connection between Trimethoprim-Sulfamethoxazole Use and Resistance in Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Antimicrobial Agents and Chemotherapy 52: 2480-5. doi:10.1128/AAC.01118-07

More like this

A recent article that examined the relationship between antibiotic use and antibiotic resistance in Finland made me realize one very sad fact: what is easy to do in Finland is nearly impossible in the U.S. because we lack a national healthcare system (note: I'm not talking about how healthcare is…
Yes, this is O157:H7, not ExPEC. Bully for you. One thing regarding popular accounts of antibiotic resistance I've noticed is that there is an overemphasis on the evolution of resistance, and an underemphasis on the spread of resistant bacteria. While the evolution of resistance is important,…
How antibiotic resistant your E. coli are has to do with where you've been, not what you eat. A recent study isolated E. coli from 567 newly hospitalized patients and 100 vegetarians (one E. coli isolate per person), and screened them for resistance to X antibiotics, including ciprofloxacin,…
Recently, I described how the most frequent killer of children in the developing world wasn't 'the Big Three' (AIDS, malaria, and TB), but boring old pneumonia. Well, the second greatest killer is diarhhea. And several factors are leading to increased treatment failure of diarhhea. Actually, it's…