An entire bacterial genome discovered inside that of a fruit fly

This article is reposted from the old Wordpress incarnation of Not Exactly Rocket Science.

Blogging on Peer-Reviewed ResearchA humble species of fruit fly is the genetic equivalent of a Russian doll - peer inside its DNA and you will see the entire genome of a species of bacteria hidden within.

Wolbachia in yellow infecting insect cells in red.The bacteria in question is Wolbachia, the most successful parasite on earth and infects about 20% of the world's species of insects. It's a poster child for selfishness. To further its own dynasty, it has evolved a series of remarkable techniques for ensuring that it gets passed on from host to host. Sometimes it gives infected individuals the ability to reproduce asexually; at other times, it does away with an entire gender.

Now, Julie Dunning-Hotopp from the J. Craig Venter Institute and Michael Clark from the University of Rochester have found an even more drastic strategy used by Wolbachia to preserve its own immortality - inserting its entire genome wholesale into that of another living thing.

Among bacteria, such gene swaps are run-of-the-mill. Humans and other multi-celled creatures must (mostly) contend ourselves with passing our genes to our young but bacteria have no such limits. They can exchange genes as easily as we exchange emails and this free trade in DNA, formally known as 'horizontal gene transfer', allows them to swap beneficial adaptations such as drug resistance genes.

Gene transfer between bacteria and eukaryotes is rare but if any bacteria was well placed to do it, it would be Wolbachia. It infects the developing sex cells of its hosts and gets passed on from mother to child in the egg itself - a prime location for integrating its genes into those of the next generation.

Drosophila ananassae has a stowaway in its genome.Other labs had already managed to detect traces of Wolbachia genes in a species of beetle and a nematode worm. To discover the full extent of its genetic infiltration, Dunning-Hotopp and Clark decided to search for Wolbachia genes in a wide range of invertebrates.

The fruit fly Drosophila ananassae quickly emerged as a promising candidate, and the duo tried to isolate its genome by killing off the infecting Wolbachia with a simple antibiotic.

It didn't work. Even with the antibiotic treatment, they still found traces of Wolbachia genes amidst the fly's DNA.

For months, the same thing happened and as frustration settled in, Dunning-Hotopp and Clark realised that the fly's cells had no living Wolbachia in them. The immigrant genes kept on turning up because they were actually part of the Drosophila genome and Clark eventually found them on the second chromosome.

Astonishingly, they found that almost all of the bacterium's genes had been transferred across. The insert is about the right size, and when Dunning-Hotopp and Clark tested for 45 genes spread throughout the Wolbachia's genome, they found 44 in the fly.

Through further investigation, they confirmed that the genes didn't just come from a few resistant Wolbachia contaminating the samples. They could be passed down from father to children, something that the parasite itself cannot do for it only passes down the female line.

Wolbachia in yellow infecting a Drosophila ovary.Dunning-Hotopp's and Clark's discovery suggests that gene transfers between bacteria and more complex creatures may be much more common than previously thought. After all, in addition to D.ananassae, the team also found smaller Wolbachia fragments in six other species of insect and four species of nematode worm.

But to truly appreciate the full scale of gene transfer from bacteria to complex organisms, we need to completely rethink the way that we sequence genomes.

In the past, genome sequencing projects have discarded traces of bacterial genes because the scientists thought they were contaminations, the odd bacteria that got mixed up in the sample. And that has almost certainly led to mistakes. Case in point: the publicly available full sequence of D.ananassae doesn't include any Wolbachia genes, because they were left out.

This isn't a mistake that we can make lightly, for this process could be a major driving force in evolution. After all, Wolbachia is the world's most common parasite and it infects the world's most numerous animal groups. Its inserted genes could rapidly provide the host animal with new genes and new abilities.

Certainly, in the fly, the Wolbachia genes are not quiet stowaways and 2% are actually switched on. Whether they actually do anything is another matter - their gene transcripts are up to a million times less common than the flies' own. Their role is a question that the researchers have yet to answer.

Nonetheless, for Wolbachia, the strategy (which Dunning-Hotopp and Clark believe was accidental) has paid off. The duo found the Wolbachia genes in D.ananassae specimens from Hawaii, Malaysia, Indonesia and India, almost unchanged. From a single ancestor that inveigled its way into the fly's genome, Wolbachia gained a free ride across the world.

Reference: Dunning-Hotopp, Clark, Oliveira, Foster, Fischer, Torres, Giebel, Kumar, Ishmael, Wang, Ingram, Nene, Shepard, Tomkins, Richards, Spiro, Ghedin, Slatko, Tettelin & Werren. Widespread lateral gene transfer from intracellular bacteria to multicellular eukaryotes. Science doi:10.1126/science.1142490

More on horizontal gene transfer: 


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Why haven't I heard of this before?

Yet more proof, if any is needed, that the planet belongs to the bacteria. The rest of us (fruit flies and humans) just happen to live here.

Seriously, if genome sequencers have been routinely discarding bacteria genes then what is the likelihood that this sort of gene transfer is going on pretty much everywhere?

The mind boggles at the possible implications to genetics, evolution and biology.

By NoAstronomer (not verified) on 30 Jul 2009 #permalink

Fascinating stuff, and further indication that our understanding of genomics is still in a primitive, infantile stage, and assuming otherwise is fraught with complications.

I remember being taught about Wolbachia once at uni. There was a group studying how it infected ladybirds, and lead to sex-dependant larvae death. It was passed on from the mother and basically killed all the male offspring. The female babies could tthen eat their dead brothers, gain more energy for survival and be more likely to pass the bacteria on.

Seriously, if genome sequencers have been routinely discarding bacteria genes then what is the likelihood that this sort of gene transfer is going on pretty much everywhere?

Wolbachia and similar bacteria appear to be limited to arthropods.

So far.

(And then, of course, "limited" is not the right term to use together with "to arthropods".)

By David MarjanoviÄ (not verified) on 31 Jul 2009 #permalink

...And nematodes. And I forgot what else.

By David MarjanoviÄ (not verified) on 31 Jul 2009 #permalink

Is viral DNA being discarded too? If so, another mechanism for genome evolution (including transduction of mammalian genes via viral transcripts) could be being lost.

By Shawn White (not verified) on 04 Aug 2009 #permalink

...not just mammalian genes...sorry, personal bias.

By shawn white (not verified) on 04 Aug 2009 #permalink