Well I finaly synthesized and purified the magic protein.
Yesterday I used this protein in an experiment that was dictated to me by the ghost of Ockham. I should know the result this afternoon.
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So which one is your protein of interest, the higher molecular weight doublet or the larger band of low molecular weight protein?
The low one. It's a bit contaminated with that upper doublet, but if you consider that the bottom band has a lower MW and thus a higher mole/mass ratio then it's not so bad. Probably 95% pure.
More pure. Less magic.