Tris (One of the few chemicals we go through in buckets)

Tris is one of the most common buffers out there and absolutely ubiquitous in molecular biology. The idea behind a buffer is that you have a compound that takes on a proton (hydrogen) at a certain pH, usually somewhere near neutrality, you have about half with and half without a proton, and you have a solution held near that pH. Tris buffers a shade above pH 8, which is a little basic, but not so bad. It's pretty transparent to UV light, water soluble, and cheap. The last is probably the big thing; we have a paint-bucket sized container of tris in the lab. It's probably the only compound I dispense with a big ol' scoop.

For reasons that are in part historical, tris is ubiquitous in DNA electrophoresis - "gels." Not to say that some people suggest other things. The two most common electrophoresis buffers are TAE (tris-acetate-EDTA) and TBE (tris-borate-EDTA); these people suggest that just borate is plenty (and faster and better, as the brand name would indicate). I've tried using the borate-only buffers with not much luck (but apparently they're not good for the larger DNAs I was trying to characterize). Post all your gel buffer rants below.

i-7520873f690f945c6f499b6048fb57c3-tris.gif

Tags

More like this

Buffers are a bit tricky. In biology, a buffer contains at least one ingredient: something to set the pH. This means having something that ionizes (takes on or loses a proton) at about the pH you want. You can set the pH within about one unit of this value (the pKa).
Last week, I promised to continue my discussion of ropes. I'm going to break that promise. But it's in a good cause.
From the President who brought you "Clean Skies (cough)" and "Healthy Forests (not)" comes a slashing of the "Buffer Zone" rule which is supposed to prohibit mining companies fr
Photons can carry enormous amount of information, but one of the problems in using them to encode information is that they are difficult to store for even short periods (they are moving at the speed of light after all!).