Tips for Yeast

i-1743f2becf4ec46cdb1caa47303d2936-yeast.jpg
No this is not an entry on shmooing, but on practical tips on working with Saccharomyces cerevisiae.

(OK confession, I have worked on yeast briefly as a rotating student in Liza Pon's Lab at Columbia.)

Much of modern cell biology is based on Yeast genetic screens, the most famous are those performed by Leland H. Hartwell and Paul Nurse to determine genes necessary for cells to divide properly (the CDC, or cell division cycle, mutants).

The only tip I can give you is to pay attention to what is done in yeast, the rule of thumb is that the yeast field is 1-2 years ahead of the rest.

That being said there are good entries on the Saccharomyces blog by dlamming about yeast in general:

Yeast Tips Vol I
Yeast Tips Vol II
The most useful site in the internet (after PubMed): http://www.yeastgenome.org/
In addition, here is a very useful link (from Saccharomyces) to Yeast Idiosyncracies: An Accumulation of Miscellaneous Observations by Cora Styles

(And remember when in doubt chant: "The Awesome Power of Yeast Genetics")

Tags

More like this

I don't know if any DIY biologists are looking for projects, but I think engineering yeast with a gene to detect heavy metals might be a good DIY biology project and I have some ideas for how to do this. What are the advantages of using yeast and working on this kind of problem? This could have a…
Yesterday I saw Dr. Alan Cowman give a talk. He's a big guy in the Plasmodium field. Plasmodium is strange. It's the eukaryote parasite that causes malaria. But that's not why it's strange. Let's put it this way, if animals, plants and fungi are three siblings, Plasmodium would be their 6th cousin…
My labmate Bruno's newest paper, "A synthetic circuit for selectively arresting daughter cells to create aging populations" came out today in the journal Nucleic Acids Research (and it's open access!). Using a cleverly designed genetic circuit that activates cell growth arrest in newly divided…
I just read this fascinating theory that was fully explained in a review that appeared in the latest issue of Cell. This theory connects the origin of cell polarity with aging and it suggests that the centrosome may carry genetic information. Today I'll focus on the first deep connection polarity…