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  2. Tips for Yeast

Tips for Yeast

By apalazzo on April 4, 2006.

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No this is not an entry on shmooing, but on practical tips on working with Saccharomyces cerevisiae.

(OK confession, I have worked on yeast briefly as a rotating student in Liza Pon's Lab at Columbia.)

Much of modern cell biology is based on Yeast genetic screens, the most famous are those performed by Leland H. Hartwell and Paul Nurse to determine genes necessary for cells to divide properly (the CDC, or cell division cycle, mutants).

The only tip I can give you is to pay attention to what is done in yeast, the rule of thumb is that the yeast field is 1-2 years ahead of the rest.

That being said there are good entries on the Saccharomyces blog by dlamming about yeast in general:

Yeast Tips Vol I
Yeast Tips Vol II
The most useful site in the internet (after PubMed): http://www.yeastgenome.org/
In addition, here is a very useful link (from Saccharomyces) to Yeast Idiosyncracies: An Accumulation of Miscellaneous Observations by Cora Styles

(And remember when in doubt chant: "The Awesome Power of Yeast Genetics")

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lab life

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