Time for a therapy session.
Man I hate this. My cortisol levels must be through the roof. I tested my clones and got weird results. I was racking my brain all weekend trying to figure out why. Then yesterday I tested a positive control, and got negative results. I feel like I'm trapped in the twilight zone. (I would have rather been at the ASCB meeting.)
What to do?
One advice that I got long time ago - if things don't work anymore, throw away all your reagents and start all over. It's the best advice that anyone has ever given me.
So today I'm doing exactly that.
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