Each row of colonies represents one yeast strain lacking one gene from the Saccharomyces cerevisiae genome. There are 8 rows per dish, and 550 dishes, resulting in 4400 strains. Saccharomyces cerevisiae has about 6000 coding genes. Strains lacking essential genes are not included for obvious reasons.
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As my wife asked me on our first date, "Is it really that small?"
She didn't really say that, but looking at that visual rep of any genome makes it seem so tiny and inconsequential.
Ok...time for more bourbon.
You could also use the Tags in the place of each ORF and screen your phenotype in the whole population of deletions at the same time.
What about 46 96-well plates instead?
Nah, I like it old school! (I'm serious).
Toothpick power!
The postdoc who performed this screen was going to use a pipetting machine to plate out a modified collection of yeast knockouts. Unforetunately he ran into problems and had to plate the cells manually.
I hope they are sealed with a parafilm or something, otherwise I can't even imagine how the room/fridge smell :|
Like a brewery probably!
Not the worst smelling microbe on a plate imo