Each row of colonies represents one yeast strain lacking one gene from the Saccharomyces cerevisiae genome. There are 8 rows per dish, and 550 dishes, resulting in 4400 strains. Saccharomyces cerevisiae has about 6000 coding genes. Strains lacking essential genes are not included for obvious reasons.
- Log in to post comments
More like this
When we think about cooperative behaviour, most of us would think of animals like ants, meerkats, lions or, indeed, humans. But don't rule out yeast. The small, single-celled fungus has provided us with much of our knowledge of genetics and molecular biology and now, it's shedding light on the…
There's lots of cool stuff coming out in the speciation literature. The Questionable Authority has posted on two recent studies on sympatric speciation (see here and here). Nature, which published the two sympatric speciation papers, has a summary available here. I am of the opinion that most…
Over the last few years it has become increasing clear that gene expression is partially regulated at the mRNA level.
What do I mean by that?
In eukaryotic cells, the first step of gene expression occurs in the nucleus when regions of DNA are transcribed into RNA. These "transcripts" then…
In a laboratory at Aberystwyth University, Wales, a scientist called Adam is doing some experiments. He is trying to find the genes responsible for producing some important enzymes in yeast, and he is going about it in a very familiar way. Based on existing knowledge, Adam is coming up with new…
As my wife asked me on our first date, "Is it really that small?"
She didn't really say that, but looking at that visual rep of any genome makes it seem so tiny and inconsequential.
Ok...time for more bourbon.
You could also use the Tags in the place of each ORF and screen your phenotype in the whole population of deletions at the same time.
What about 46 96-well plates instead?
Nah, I like it old school! (I'm serious).
Toothpick power!
The postdoc who performed this screen was going to use a pipetting machine to plate out a modified collection of yeast knockouts. Unforetunately he ran into problems and had to plate the cells manually.
I hope they are sealed with a parafilm or something, otherwise I can't even imagine how the room/fridge smell :|
Like a brewery probably!
Not the worst smelling microbe on a plate imo