However, unlike the MRI (which had strong personal significance), this time the sequencing data, hung by the lamp to the right, is of nothing in particular. Thanks everyone for the comments - it was interesting and also valuable. Nice to know that readers appreciate the nuances in scenarios such as this. Nuances are important in so many things.
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Wow! I didn't know people used gels any more!
I have some of old autorads hanging around to show students and software engineers, but I assumed that everyone, today, used fluorescent labeling and genetic analyzers.
We do a manual gel experiment in one of the labs I teach, primarily because students have always thought it was pretty cool to do it old school (i.e. students would always say to keep it in the syllabus). I figure we can get away with not doing the more up to date cycle sequencing, because this lab course has upteem other PCR based experiments already.
That makes sense.
I didn't want to mess with getting a license for using radioactivity, so I used to have my students do their sequencing reactions with a biotinylated primer, transfer the DNA from their gels to a filter, and detect the DNA with anti-biotin antibodies conjugated to alkaline phosphatase, and the substrate.
Radioactivity would made it all sooooo much easier.
you guys are making those words up, right? i get it, i get it. very funny, biologists.
Sandra, Don't mind Ben - he's, like an "engineer" or something.
Ben,
I never make up words. That's why we hire marketing and business people!